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Part:BBa_K2371016:Design

Designed by: Wang Xiao   Group: iGEM17_BGIC-Union   (2017-10-24)


C-Fluc-dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1689
    Illegal NheI site found at 4876
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 30
    Illegal BamHI site found at 3968
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 353
    Illegal AgeI site found at 492
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

unaccomplished...


Source

luciferase from S30 cell-free system kit from promega ; dCas9 from plasmid PX335

References